Selected article for: "additional file and gene expression"
Author: Lindqvist, Richard; Mundt, Filip; Gilthorpe, Jonathan D.; Wölfel, Silke; Gekara, Nelson O.; Kröger, Andrea; Överby, Anna K.
Title: Fast type I interferon response protects astrocytes from flavivirus infection and virus-induced cytopathic effects
  • Document date: 2016_10_24
    • ID: 09tcnsxv_39
    Snippet: Our data indicate that WT astrocytes are quite resistant to viral infection and that the main effectors in the supernatant from virus-infected astrocytes are type I IFNs. To characterize these responses in more detail, we analyzed transcriptional regulation by RNAseq. Gene expression levels from uninfected IFNAR −/− astrocytes and IFNαB/D-treated, or supernatant-treated, WT astrocytes were compared to untreated WT astrocytes. Fold-change val.....
    Document: Our data indicate that WT astrocytes are quite resistant to viral infection and that the main effectors in the supernatant from virus-infected astrocytes are type I IFNs. To characterize these responses in more detail, we analyzed transcriptional regulation by RNAseq. Gene expression levels from uninfected IFNAR −/− astrocytes and IFNαB/D-treated, or supernatant-treated, WT astrocytes were compared to untreated WT astrocytes. Fold-change values of at least twofold over the uninfected control and a q value of <0.05 were considered indicative of upor downregulation. Our data yielded 732 upregulated transcripts while 944 were found to be downregulated in IFNAR −/− astrocytes. Treatment of primary astrocytes with IFNαB/D or supernatant led to 634 or 1092 upregulated and 149 or 423 downregulated genes, respectively (Additional file 1: Table S1 ).

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