Author: Joyjinda, Yutthana; Rodpan, Apaporn; Chartpituck, Pongtorn; Suthum, Krairerk; Yaemsakul, Suphaluk; Cheun-Arom, Thaniwan; Bunprakob, Saowalak; Olival, Kevin J.; Stokes, Martha M.; Hemachudha, Thiravat; Wacharapluesadee, Supaporn
Title: First Complete Genome Sequence of Human Coronavirus HKU1 from a Nonill Bat Guano Miner in Thailand Document date: 2019_2_7
ID: 10r5p0nu_2
Snippet: The nasopharyngeal swab found CoV positive by conventional PCR was subjected to metagenomic sequencing on the Illumina MiSeq platform. RNA was extracted using a QIAamp viral RNA mini kit (Qiagen, Germany), followed by DNase treatment. A DNA library was prepared using a TruSeq total RNA with Ribo-zero globin kit (Illumina). The quality and quantity of the DNA library was estimated by using the QIAxcel Advanced system and QIASeq library quantificat.....
Document: The nasopharyngeal swab found CoV positive by conventional PCR was subjected to metagenomic sequencing on the Illumina MiSeq platform. RNA was extracted using a QIAamp viral RNA mini kit (Qiagen, Germany), followed by DNase treatment. A DNA library was prepared using a TruSeq total RNA with Ribo-zero globin kit (Illumina). The quality and quantity of the DNA library was estimated by using the QIAxcel Advanced system and QIASeq library quantification kit, respectively (Qiagen, Germany). The 17 pmol of DNA library was injected into the flow cell and sequenced using a MiSeq reagent kit version 3.
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