Author: Frey, Kenneth G.; Redden, Cassie L.; Bishop-Lilly, Kimberly A.; Johnson, Reed; Hensley, Lisa E.; Raviprakash, Kanakatte; Luke, Thomas; Kochel, Tad; Mokashi, Vishwesh P.; Defang, Gabriel N.
Title: Full-Genome Sequence of Human Betacoronavirus 2c Jordan-N3/2012 after Serial Passage in Mammalian Cells Document date: 2014_5_29
ID: 0b2blj2c_3
Snippet: Total RNA was extracted from the supernatants of infected cell cultures, and sequencing libraries were created using the TruSeq RNA sample prep version 2 kit (Illumina, Inc.), beginning the protocol at the fragmentation step. Paired-end 151-base sequencing was performed on the MiSeq (average Ͼ730 Mb/sample). The reads were aligned against the NCBI reference (accession no. KC776147.1) using the CLC bio Genomics Workbench (version 6.5). In additio.....
Document: Total RNA was extracted from the supernatants of infected cell cultures, and sequencing libraries were created using the TruSeq RNA sample prep version 2 kit (Illumina, Inc.), beginning the protocol at the fragmentation step. Paired-end 151-base sequencing was performed on the MiSeq (average Ͼ730 Mb/sample). The reads were aligned against the NCBI reference (accession no. KC776147.1) using the CLC bio Genomics Workbench (version 6.5). In addition, the sequence reads were de novo assembled using CLC bio.
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