Selected article for: "buffer saline and monoclonal antibody"
Author: Lu, Shuai; Chen, Yingzhu; Qin, Kun; Zhou, Jianfang; Lou, Yongliang; Tan, Wenjie
Title: Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in China
  • Document date: 2016_4_15
    • ID: 1ghgd7yg_30
    Snippet: The purified rNPs of CCoV or CRCoV separated by electrophoresis on the gels were transferred to nitrocellulose membrane, followed by blocking in 5% skim milk in phosphate buffer saline (PBS) at room temperature for 2 h. Afterwards, the blots were washed and incubated with anti-His monoclonal antibody (1 : 1,000 dilution) and mice antisera against HCoV-229E, NL63, OC43, HKU1 and MERS-CoV (1 : 200 dilution) separately at 4°C overnight. After three.....
    Document: The purified rNPs of CCoV or CRCoV separated by electrophoresis on the gels were transferred to nitrocellulose membrane, followed by blocking in 5% skim milk in phosphate buffer saline (PBS) at room temperature for 2 h. Afterwards, the blots were washed and incubated with anti-His monoclonal antibody (1 : 1,000 dilution) and mice antisera against HCoV-229E, NL63, OC43, HKU1 and MERS-CoV (1 : 200 dilution) separately at 4°C overnight. After three washes, goat anti-mouse IgG conjugated with HRP (1 : 5,000 dilution, ZSGB-Bio) or IRDye anti-mouse IgG (1 : 5,000 dilution, Odyssey) was added respectively and incubated at room temperature for 2 h. The membrane was thoroughly washed and then developed with the substrate DAB or using the Odyssey TM infrared imaging system.

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