Selected article for: "close proximity and confocal microscopy"

Author: Kummer, Susann; Avinoam, Ori; Kräusslich, Hans-Georg
Title: IFITM3 Clusters on Virus Containing Endosomes and Lysosomes Early in the Influenza A Infection of Human Airway Epithelial Cells
  • Document date: 2019_6_12
  • ID: 1345qct4_5
    Snippet: We analyzed the localization of endogenous IFITM3 through the course of IAV infection using confocal and super-resolution (STED) microscopy. We observed a strong clustering of IFITM3 early after IAV infection, which was distinct from the interferon alpha induced IFITM3 up-regulation. Moreover, a two-color STED nanoscopy revealed a close proximity of IFITM3 with the IAV NP protein in Rab11 positive compartments within a time range of 1-6 h after t.....
    Document: We analyzed the localization of endogenous IFITM3 through the course of IAV infection using confocal and super-resolution (STED) microscopy. We observed a strong clustering of IFITM3 early after IAV infection, which was distinct from the interferon alpha induced IFITM3 up-regulation. Moreover, a two-color STED nanoscopy revealed a close proximity of IFITM3 with the IAV NP protein in Rab11 positive compartments within a time range of 1-6 h after the IAV addition.

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