Selected article for: "Golgi apparatus and large complex"
Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein
  • Document date: 2016_7_6
    • ID: 1aptufp6_24
    Snippet: The first signs of disruption of Golgi morphology largely began during or even preceding the accumulation of visible 3A signal (Fig. 5B , white asterisk in the 3A-GFP channel; also see Movie S1 in the supplemental material), suggesting that local changes to morphology occur rapidly and may be triggered by 3A accumulation in regions of the Golgi apparatus outside the imaging plane. To facilitate the detection of the whole Golgi complex and to moni.....
    Document: The first signs of disruption of Golgi morphology largely began during or even preceding the accumulation of visible 3A signal (Fig. 5B , white asterisk in the 3A-GFP channel; also see Movie S1 in the supplemental material), suggesting that local changes to morphology occur rapidly and may be triggered by 3A accumulation in regions of the Golgi apparatus outside the imaging plane. To facilitate the detection of the whole Golgi complex and to monitor large-scale changes in Golgi morphology, imaging was carried out using a wider confocal pinhole (600 m). Global Golgi fragmentation typically began 10 to 30 min after 3A started to accumulate in large amounts (Fig. 6 ) (n Ï­ 8; range, 0 to 45 min), which presumably reflects the time taken for the cumulative local changes to become apparent within the entire structure, and was completed 75 to 105 min after the initial detection of 3A-GFP signal (n Ï­ 8). Together, these data

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