Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein Document date: 2016_7_6
ID: 1aptufp6_35
Snippet: In conclusion, we have presented split-GFP-tagged CVB3 as a new tool for the direct visualization of enterovirus ROs in living cells, which will allow the study of their dynamics. Furthermore, the combination of live-cell imaging with CLEM will enable us to establish a direct link between events observed by fluorescence microscopy and morphologically distinct ROs, which could shed light onto the possible differentiated roles of these different vi.....
Document: In conclusion, we have presented split-GFP-tagged CVB3 as a new tool for the direct visualization of enterovirus ROs in living cells, which will allow the study of their dynamics. Furthermore, the combination of live-cell imaging with CLEM will enable us to establish a direct link between events observed by fluorescence microscopy and morphologically distinct ROs, which could shed light onto the possible differentiated roles of these different virus-induced structures in enterovirus infection. This system will therefore serve as a valuable tool for future studies on the origin and function of enterovirus ROs.
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