Author: He, Chaobin; Wang, Jun; Sun, Shuxin; Zhang, Yu; Li, Shengping
Title: Immunomodulatory Effect after Irreversible Electroporation in Patients with Locally Advanced Pancreatic Cancer Document date: 2019_5_12
ID: 1pbzbydu_8
Snippet: . . Sample Collection. All blood samples were collected before the hypothesis of this study was known. The blood samples were collected using Na-heparin plasma tubes from enrolled patients before IRE (preOP) and then on days 3 (D3) and 7 (D7) after IRE. Isolation of peripheral blood mononuclear cells (PBMCs) was processed immediately using Hypaque-Ficoll (Promega) and frozen in liquid nitrogen in 5% (v/v) plus 95% (v/v) autologous serum [20] ......
Document: . . Sample Collection. All blood samples were collected before the hypothesis of this study was known. The blood samples were collected using Na-heparin plasma tubes from enrolled patients before IRE (preOP) and then on days 3 (D3) and 7 (D7) after IRE. Isolation of peripheral blood mononuclear cells (PBMCs) was processed immediately using Hypaque-Ficoll (Promega) and frozen in liquid nitrogen in 5% (v/v) plus 95% (v/v) autologous serum [20] .
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