Author: Jordana-Lluch, Elena; Giménez, Montserrat; Quesada, M. Dolores; Ausina, Vicente; Martró, Elisa
Title: Improving the Diagnosis of Bloodstream Infections: PCR Coupled with Mass Spectrometry Document date: 2014_4_9
ID: 05zahl5n_24
Snippet: An advantage of the PCR/ESI-MS assay relative to the MALDI-TOF assay is that PCR/ESI-MS has been optimized to achieve a rapid diagnosis from direct clinical specimens. To date, two clinical evaluations of the PCR/ESI-MS for the diagnosis of bloodstream infections from whole blood have been published. Jordana-Lluch et al. [21] evaluated this system analyzing 247 whole blood specimens (75 with a paired positive blood culture and 172 with a negative.....
Document: An advantage of the PCR/ESI-MS assay relative to the MALDI-TOF assay is that PCR/ESI-MS has been optimized to achieve a rapid diagnosis from direct clinical specimens. To date, two clinical evaluations of the PCR/ESI-MS for the diagnosis of bloodstream infections from whole blood have been published. Jordana-Lluch et al. [21] evaluated this system analyzing 247 whole blood specimens (75 with a paired positive blood culture and 172 with a negative blood culture result), and Laffler et al. [22] tested 464 whole blood specimens with a positive paired blood culture and 442 with a negative blood culture result. The agreement between blood culture followed by biochemical identification and PCR/ESI-MS was good in both studies: 77.1% in the Jordana-Lluch et al. study [21] and 78.6% in the Laffler et al. study [22] .
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