Author: Natalia Fintelman-Rodrigues; Carolina Q. Sacramento; Carlyle Ribeiro Lima; Franklin Souza da Silva; André C. Ferreira; Mayara Mattos; Caroline S. de Freitas; Vinicius Cardoso Soares; Suelen da Silva Gomes Dias; Jairo R. Temerozo; Milene Miranda; Aline R. Matos; Fernando A. Bozza; Nicolas Carels; Carlos Roberto Alves; Marilda M. Siqueira; Patrícia T. Bozza; Thiago Moreno L. Souza
Title: Atazanavir inhibits SARS-CoV-2 replication and pro-inflammatory cytokine production Document date: 2020_4_5
ID: 6zk0ioep_37
Snippet: Monolayers of Vero cells (2 x 10 4 cell/well) in 96-well plates were infected with a log-based dilution of supernatants containing SARS-CoV-2 for 1h at 37°C. Cells were washed, fresh medium added with 2% FBS and 3 to 5 days post infection the cytopathic effect was scored in at least 10 replicates per dilution by independent readers. The reader was blind with respect to source of the supernatant. A Reed and Muench scoring method was employed to d.....
Document: Monolayers of Vero cells (2 x 10 4 cell/well) in 96-well plates were infected with a log-based dilution of supernatants containing SARS-CoV-2 for 1h at 37°C. Cells were washed, fresh medium added with 2% FBS and 3 to 5 days post infection the cytopathic effect was scored in at least 10 replicates per dilution by independent readers. The reader was blind with respect to source of the supernatant. A Reed and Muench scoring method was employed to determine TCID 50 /mL 42 .
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