Selected article for: "different concentration and gel system"
Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate
  • Document date: 2018_5_16
    • ID: 1ghbutov_22
    Snippet: The activity of PT-1 on the formation of HIV-1 gp41 six-helix bundle was measured by native polyacrylamide gel electrophoresis (N-PAGE) assay. In general, 30 µM N-peptide N36 (synthesized by Beijing Scilight Biotechnology Ltd., Beijing, China) was incubated with different concentration of PT-1 at 37 • C for 30 min, then 40 µM C34 peptide was added for another 30 min of incubation, and the samples were mixed with Tris-glycine native sample buf.....
    Document: The activity of PT-1 on the formation of HIV-1 gp41 six-helix bundle was measured by native polyacrylamide gel electrophoresis (N-PAGE) assay. In general, 30 µM N-peptide N36 (synthesized by Beijing Scilight Biotechnology Ltd., Beijing, China) was incubated with different concentration of PT-1 at 37 • C for 30 min, then 40 µM C34 peptide was added for another 30 min of incubation, and the samples were mixed with Tris-glycine native sample buffer and loaded onto 18% Tris-glycine gel for electrophoresis at 120 V constant voltage. After 90 min electrophoresis, the gel was stained with Coomassie Blue dyes and imaged with Bio-Rad Gel Doc XR + Imaging System (Bio-Rad, Hercules, CA, USA). AZT was used as a negative control.

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