Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate Document date: 2018_5_16
ID: 1ghbutov_26
Snippet: The inhibitory effect of PT-1 on reverse transcriptase (RT) was measured using commercial colorimetric assay (Roche, Berlin, Germany) following the manufacturer's instructions. Briefly, PT-1 (0.7, 3.5, and 17.6 µM) were incubated with 4 ng HIV-1 RT, digoxigenie (DIG) and biotin-labeled DNA solution per reaction tube for 1 h at 37 • C. The mixture was then incubated with a streptavidin coated surface of a microplate for 1 h at 37 • C. After e.....
Document: The inhibitory effect of PT-1 on reverse transcriptase (RT) was measured using commercial colorimetric assay (Roche, Berlin, Germany) following the manufacturer's instructions. Briefly, PT-1 (0.7, 3.5, and 17.6 µM) were incubated with 4 ng HIV-1 RT, digoxigenie (DIG) and biotin-labeled DNA solution per reaction tube for 1 h at 37 • C. The mixture was then incubated with a streptavidin coated surface of a microplate for 1 h at 37 • C. After extensively washing, DIG-labeled DNA was co-incubated with the peroxidase conjugated anti-DIG-POD antibody for 1 h. Later, 200 µL 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) substrate was added to each well after washing, and after 30 min of incubation, the absorbance of the cleaved product was measured at OD 405 nm and the percent inhibition was calculated as compared to the no inhibitor control, with nevirapine (NVP) used as a positive control.
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