Author: Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi
Title: Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate Document date: 2018_5_16
ID: 1ghbutov_28
Snippet: The inhibitory effect of PT-1 on HIV-1 protease was measured by commercial fluorimetric assay (AnaSpec, Fremont, CA, USA) according to the manufacturer's instructions. In brief, PT-1 was first incubated with HIV-1 protease in an assay buffer for 15 min, then the mixture was incubated with a HIV-1 protease substrate solution for 60 min at room temperature. The fluorescence intensity was measured at Ex/Em = 490 nm/520 nm. Pepstatin A was used as a .....
Document: The inhibitory effect of PT-1 on HIV-1 protease was measured by commercial fluorimetric assay (AnaSpec, Fremont, CA, USA) according to the manufacturer's instructions. In brief, PT-1 was first incubated with HIV-1 protease in an assay buffer for 15 min, then the mixture was incubated with a HIV-1 protease substrate solution for 60 min at room temperature. The fluorescence intensity was measured at Ex/Em = 490 nm/520 nm. Pepstatin A was used as a positive control.
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