Author: Kun Sun; Liuqi Gu; Li Ma; Yunfeng Duan
Title: Atlas of ACE2 gene expression in mammals reveals novel insights in transmission of SARS-Cov-2 Document date: 2020_3_31
ID: 89sg0cpk_15
Snippet: Considering that the reference genomes and gene annotations for most of the species included in this study are far from complete, in order to avoid potential biases of the conventional FPKM (Fragments per Kilobase Million) values, we used ACTB (Actin Beta) gene from each RNA-seq experiment to normalize ACE2 expression for . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which wa.....
Document: Considering that the reference genomes and gene annotations for most of the species included in this study are far from complete, in order to avoid potential biases of the conventional FPKM (Fragments per Kilobase Million) values, we used ACTB (Actin Beta) gene from each RNA-seq experiment to normalize ACE2 expression for . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.30.015644 doi: bioRxiv preprint 7 appropriate comparisons across species and tissue types. ACTB is a housekeeping gene that is abundantly and stably expressed in most cell types, and is commonly used as an internal control for gene expression normalizations 31 . In addition, ACTB gene is also conserved in all the species investigated in this study. The following formula was used to calculate normalized ACE2 expression:
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