Author: Suddala, Krishna C.; Lee, Christine C.; Meraner, Paul; Marin, Mariana; Markosyan, Ruben M.; Desai, Tanay M.; Cohen, Fredric S.; Brass, Abraham L.; Melikyan, Gregory B.
Title: Interferon-induced transmembrane protein 3 blocks fusion of sensitive but not resistant viruses by partitioning into virus-carrying endosomes Document date: 2019_1_14
ID: 15wxk8lt_51
Snippet: The pR8ΔEnv, pR9ΔEnv, BlaM-Vpr, pcRev, pMDG-VSV-G, MLV-Gag-Pol, HIV-1 Gag-mCherry (encoding an uncleavable mCherry fluorescent tag used in fixed cell co-localization experiments), IFITM3/pQCXIP, F75/78A IFITM3/pQCXIN expression vectors were described previously [15, 21, 43, 75] . The mCherry-2xCL-YFP-Vpr (mCherry fused to YFP-Vpr through a cleavable linker containing two HIV protease cleavage sites-2xCL), as described previously [50] , was used.....
Document: The pR8ΔEnv, pR9ΔEnv, BlaM-Vpr, pcRev, pMDG-VSV-G, MLV-Gag-Pol, HIV-1 Gag-mCherry (encoding an uncleavable mCherry fluorescent tag used in fixed cell co-localization experiments), IFITM3/pQCXIP, F75/78A IFITM3/pQCXIN expression vectors were described previously [15, 21, 43, 75] . The mCherry-2xCL-YFP-Vpr (mCherry fused to YFP-Vpr through a cleavable linker containing two HIV protease cleavage sites-2xCL), as described previously [50] , was used for single particle tracking of fusion events in live cells. The pCAGGS vectors encoding influenza H1N1 WSN HA and NA were provided by Drs. Donna Tscherne and Peter Palese (Icahn School of Medicine, Mount Sinai) [76] . The LASV GPc plasmid was a gift from Dr. F.-L. Cossett (Université de Lyon, France) [77] .
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