Author: Kumar, Satyendra; Arankalle, Vidya A.
Title: Intracranial Administration of P Gene siRNA Protects Mice from Lethal Chandipura Virus Encephalitis Document date: 2010_1_7
ID: 1mzq227n_40
Snippet: Short hairpin RNA (shRNA) validation with pAcGPF1N1-CHPV-P plasmid and with CHPV Flowcytometery and real time one step RT-PCR documented that P-2 shRNA expressed either from Pol III (pH1-P2) or Pol II promoter (pCMV-P2) was as efficient as P-2 siRNA, when the validation was done with pAcGFP1N1-CHPV-P. The reduction was approximately 70% (Fig 6a) (p,0.05) ......
Document: Short hairpin RNA (shRNA) validation with pAcGPF1N1-CHPV-P plasmid and with CHPV Flowcytometery and real time one step RT-PCR documented that P-2 shRNA expressed either from Pol III (pH1-P2) or Pol II promoter (pCMV-P2) was as efficient as P-2 siRNA, when the validation was done with pAcGFP1N1-CHPV-P. The reduction was approximately 70% (Fig 6a) (p,0.05) .
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