Isolation and Characterization of a Novel Gammaherpesvirus from a Microbat Cell Line

Shabman, Reed S.; Shrivastava, Susmita; Tsibane, Tshidi; Attie, Oliver; Jayaprakash, Anitha; Mire, Chad E.; Dilley, Kari E.; Puri, Vinita; Stockwell, Timothy B.; Geisbert, Thomas W.; Sachidanandam, Ravi; Basler, Christopher F.

Phylogenic analyses were performed on the BGHV8 glycoprotein B (gB) and v-OX2 protein sequences. For gB, a protein BLAST search using gB was performed, results were used to create alignments, and sequences were aligned and trimmed prior to generation of a tree. An outgroup, human herpesvirus 1, was used to generate a phylogenetic tree using the LG substitution model and 100 bootstraps and root it on the outgroup. For v-OX2, a protein BLAST search against all viral sequences was performed, resulting herpesvirus sequences were aligned and trimmed, and a tree was constructed using methods similar to those described for gB. A v-OX2 protein BLAST search was also performed against the mammalian protein database. Results were aligned and trimmed, and a tree was constructed using methods described above.