Author: Zhang, Qingshui; Cao, Yanxin; Wang, Jun; Fu, Guanghua; Sun, Mengxu; Zhang, Lijiao; Meng, Li; Cui, Guolin; Huang, Yu; Hu, Xueying; Su, Jingliang
Title: Isolation and characterization of an astrovirus causing fatal visceral gout in domestic goslings Document date: 2018_4_19
ID: 1k5jucae_43
Snippet: Tissue samples were prepared as a 10% suspension (w/ v) in PBS and were homogenized using beads in a highthroughput Tissuelyser (Nibo Scientz Biotechnology Co., Ltd., Zhejiang, China). Then, the homogenate was centrifuged at 8000×g for 5 min at 4°C and 150 μl of the supernatant was used for RNA extraction. Total RNA was extracted and converted to cDNA using the kits described above. PCR amplification was conducted using a set of specific prime.....
Document: Tissue samples were prepared as a 10% suspension (w/ v) in PBS and were homogenized using beads in a highthroughput Tissuelyser (Nibo Scientz Biotechnology Co., Ltd., Zhejiang, China). Then, the homogenate was centrifuged at 8000×g for 5 min at 4°C and 150 μl of the supernatant was used for RNA extraction. Total RNA was extracted and converted to cDNA using the kits described above. PCR amplification was conducted using a set of specific primers (GAstVF7/GAstVR7, Table 2 ), targeting the ORF2 gene of the isolate. For virus shedding detection, swab samples were vortexed and centrifuged. After centrifugation, 150 μl of the supernatant was processed for RT-PCR detection in the same manner.
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