Selected article for: "infection positive control and negative control"
Author: Priolo, Vito; Martínez-Orellana, Pamela; Pennisi, Maria Grazia; Masucci, Marisa; Prandi, David; Ippolito, Dorotea; Bruno, Federica; Castelli, Germano; Solano-Gallego, Laia
Title: Leishmania infantum-specific IFN-? production in stimulated blood from cats living in areas where canine leishmaniosis is endemic
  • Document date: 2019_3_26
    • ID: 04ljoezz_13
    Snippet: ELISA was performed as previously described [20, 21] . All plates included the serum from a sick cat from Cyprus with a confirmed infection with L. infantum as positive control, and the serum of a cat from an area where leishmaniosis was not endemic as a negative control. All samples were run in duplicate. The cut-off was established at 12.3 ELISA units (EU) (mean ± 3 standard deviations of sera from 81 cats from the UK, a non-endemic area)......
    Document: ELISA was performed as previously described [20, 21] . All plates included the serum from a sick cat from Cyprus with a confirmed infection with L. infantum as positive control, and the serum of a cat from an area where leishmaniosis was not endemic as a negative control. All samples were run in duplicate. The cut-off was established at 12.3 ELISA units (EU) (mean ± 3 standard deviations of sera from 81 cats from the UK, a non-endemic area).

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