Author: Warner, Nikole L.; Jokinen, Jenny D.; Beier, Juliane I.; Sokoloski, Kevin J.; Lukashevich, Igor S.
Title: Mammarenaviral Infection Is Dependent on Directional Exposure to and Release from Polarized Intestinal Epithelia Document date: 2018_2_10
ID: 1t8jmunt_12
Snippet: The attachment of viral particles was assessed quantitatively via the ∆∆Ct method [39] . Briefly, Ct values corresponding to the viral targets were normalized internally via the subtraction of the 18S rRNA levels detected within each sample. The resulting ∆Ct values of the washed tissue culture cells were then compared relative to the bound unwashed Input controls. The resulting ∆∆Ct values were used to determine the relative quantities.....
Document: The attachment of viral particles was assessed quantitatively via the ∆∆Ct method [39] . Briefly, Ct values corresponding to the viral targets were normalized internally via the subtraction of the 18S rRNA levels detected within each sample. The resulting ∆Ct values of the washed tissue culture cells were then compared relative to the bound unwashed Input controls. The resulting ∆∆Ct values were used to determine the relative quantities of viral nucleic acids in the Bound (washed) and Input (unwashed) samples; these values were then plotted, and attachment was reported as further calculated via the ratio of Basolateral/Apical attachment.
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