Author: Joshua A. Hayward; Mary Tachedjian; Adam Johnson; Tamsin B. Gordon; Jie Cui; Glenn A. Marsh; Michelle L. Baker; Lin-Fa Wang; Gilda Tachedjian
Title: Bats Possess Unique Variants of the Antiviral Restriction Factor Tetherin Document date: 2020_4_9
ID: eksi9yia_22
Snippet: To further investigate the diversity of tetherin in non-fruit bat species, we amplified the tetherin nucleotide sequences for the vesper bat species Myotis ricketti and Miniopterus schreibersii using primers designed on the basis of the predicted mRNA sequences (Table 3A) . These primers were used in a PCR analysis of cDNA samples generated from M. ricketti spleen tissue and a M. schreibersii kidney cell line to amplify the tetherin coding domain.....
Document: To further investigate the diversity of tetherin in non-fruit bat species, we amplified the tetherin nucleotide sequences for the vesper bat species Myotis ricketti and Miniopterus schreibersii using primers designed on the basis of the predicted mRNA sequences (Table 3A) . These primers were used in a PCR analysis of cDNA samples generated from M. ricketti spleen tissue and a M. schreibersii kidney cell line to amplify the tetherin coding domain sequences (Table 3A) . Primers for M. ricketti were also used to analyse cDNA generated from an M. macropus kidney cell line as it was reasoned that given that these species belong to the same genus, primers designed for one might be capable of amplifying tetherin from the other. Following PCR, amplified DNA from M. ricketti, M. macropus, and M. schreibersii, were gel purified, cloned and sequenced.
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