Author: Chen, Qi; Wang, Leyi; Zheng, Ying; Zhang, Jianqiang; Guo, Baoqing; Yoon, Kyoung-Jin; Gauger, Phillip C.; Harmon, Karen M.; Main, Rodger G.; Li, Ganwu
Title: Metagenomic analysis of the RNA fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the United States Document date: 2018_5_25
ID: 1nyhllij_5
Snippet: Two hundred and seventeen fecal samples, stored at − 80°C upon use, were selected from submissions to ISUVDL between February 2015 and February 2016. All of the fecal samples were collected from pigs with acute diarrhea, tested positive for PEDV RNA by virus-specific real-time reverse transcription PCR (rRT-PCR) with Ct values ranging 8-26, and requested for whole genome sequencing of PEDV with HTS. Fecal samples negative with PEDV rRT-PCR was.....
Document: Two hundred and seventeen fecal samples, stored at − 80°C upon use, were selected from submissions to ISUVDL between February 2015 and February 2016. All of the fecal samples were collected from pigs with acute diarrhea, tested positive for PEDV RNA by virus-specific real-time reverse transcription PCR (rRT-PCR) with Ct values ranging 8-26, and requested for whole genome sequencing of PEDV with HTS. Fecal samples negative with PEDV rRT-PCR was not included in this study as negative control. Of the 217 samples, 173 samples were submitted from swine operations in 17 US states while the remaining 44 samples were submitted without geographical information.
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