Author: Takhampunya, Ratree; Korkusol, Achareeya; Pongpichit, Chalermpol; Yodin, Komsan; Rungrojn, Artharee; Chanarat, Nitima; Promsathaporn, Sommai; Monkanna, Taweesak; Thaloengsok, Sasikanya; Tippayachai, Bousaraporn; Kumfao, Naruemon; Richards, Allen L.; Davidson, Silas A.
Title: Metagenomic Approach to Characterizing Disease Epidemiology in a Disease-Endemic Environment in Northern Thailand Document date: 2019_2_26
ID: 0gi6qzw0_31
Snippet: After performing NGS analysis of pooled samples, all samples in each NGS-positive pool for potential pathogenic bacteria were individually tested by their respective confirmatory assays using either real-time PCR or conventional PCR as indicated in Supplementary Table S1 . Any positive signal was then confirmed by DNA sequencing by the Sanger method for species characterization. The prevalence rate for each pathogen was calculated based on the nu.....
Document: After performing NGS analysis of pooled samples, all samples in each NGS-positive pool for potential pathogenic bacteria were individually tested by their respective confirmatory assays using either real-time PCR or conventional PCR as indicated in Supplementary Table S1 . Any positive signal was then confirmed by DNA sequencing by the Sanger method for species characterization. The prevalence rate for each pathogen was calculated based on the number of positive samples verified by confirmatory assays in the total number of samples studied. For some pathogens including O. tsutsugamushi and Bartonella spp., all samples were screened as routine tests. Therefore, the prevalence rate was calculated based on the number of combined positive samples detected by the NGS analysis then confirmatory assays and routine screening tests.
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