Author: Klaile, Esther; Klassert, Tilman E; Scheffrahn, Inka; Müller, Mario M; Heinrich, Annina; Heyl, Kerstin A; Dienemann, Hendrik; Grünewald, Christiane; Bals, Robert; Singer, Bernhard B; Slevogt, Hortense
Title: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons Document date: 2013_8_14
ID: 1fmsipqu_25
Snippet: NHBE cells were cultured and treated as described above, detached using trypsin/EDTA (Invitrogen/Life Science) and kept in PBS/2% FBS on ice. Cells were stained using antibodies B3-17 (CEACAM1) and 2H8-5 (CEACAM5) at 10 μg/ml, and 1:200 PE-conjugated goat anti-mouse IgG. Analysis was done using the FACSAria II (BD Biosciences) and FlowJo (Tree Star Inc.) Software......
Document: NHBE cells were cultured and treated as described above, detached using trypsin/EDTA (Invitrogen/Life Science) and kept in PBS/2% FBS on ice. Cells were stained using antibodies B3-17 (CEACAM1) and 2H8-5 (CEACAM5) at 10 μg/ml, and 1:200 PE-conjugated goat anti-mouse IgG. Analysis was done using the FACSAria II (BD Biosciences) and FlowJo (Tree Star Inc.) Software.
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