Author: Perra, Léa; Balloy, Viviane; Foussignière, Tobias; Moissenet, Didier; Petat, Hortense; Mungrue, Imran N.; Touqui, Lhousseine; Corvol, Harriet; Chignard, Michel; Guillot, Loic
Title: CHAC1 Is Differentially Expressed in Normal and Cystic Fibrosis Bronchial Epithelial Cells and Regulates the Inflammatory Response Induced by Pseudomonas aeruginosa Document date: 2018_11_29
ID: 10fe70kl_41
Snippet: The fine tuning of immune responses by the UPR has been extensively studied (37) . However, its contribution to the regulation of cytokine production in bacterial infection is not well understood (16) . Here, we observed that inhibition of CHAC1 mRNA expression by siRNA promoted IL-8 and CCL2 expression and PGE2 secretion, suggesting that CHAC1 prevents excessive inflammatory responses dependent on the NF-κB p65 pathway. These results were consi.....
Document: The fine tuning of immune responses by the UPR has been extensively studied (37) . However, its contribution to the regulation of cytokine production in bacterial infection is not well understood (16) . Here, we observed that inhibition of CHAC1 mRNA expression by siRNA promoted IL-8 and CCL2 expression and PGE2 secretion, suggesting that CHAC1 prevents excessive inflammatory responses dependent on the NF-κB p65 pathway. These results were consistent with a recent study reporting that bronchial epithelial cells undergoing ER stress showed reduced IL-8 and CXCL1 production in the presence of inflammatory stimuli (38) . Indeed, pretreatment of bronchial epithelial cells with TM [shown here and by others (11) to be able to increase CHAC1 expression] can prevent IL-8 and CXCL1 production. In contrast, inhibition of CHAC1 mRNA expression by siRNA inhibits IL-6 production, suggesting that CHAC1 may promote IL-6 expression. Our results were consistent with a previous study showing that TM promotes IL-6 secretion in bronchial IB3-1 (CF deficient) and C38 cells stimulated with flagellin (12) . Previous works have demonstrated that IL-6 production in A375 skin cells is dependent on CHOP (39) . However, CHOP is expected to be upstream in the CHAC1 pathway and does not explain our results; future studies are needed to determine how the IL-6 pathway is affected specifically in lung epithelial cells.
Search related documents:
Co phrase search for related documents- bacterial infection and epithelial cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- bacterial infection and ER stress: 1, 2, 3, 4
- bacterial infection and expression promote: 1, 2
- bacterial infection and fine tuning: 1, 2
- bacterial infection and future study: 1, 2, 3, 4
- bacterial infection and immune response: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- bacterial infection and inflammatory response: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64
- bacterial infection and lung epithelial cell: 1, 2, 3, 4
- bacterial infection and previous study: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- bacterial infection and previous work: 1, 2, 3
- bacterial infection and production inhibit: 1, 2
- bacterial infection and recent study: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- bacterial infection and skin cell: 1, 2
- CHAC1 expression and epithelial cell: 1
- CHAC1 expression and ER stress: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date