Selected article for: "buffer equal and western blot analysis"
Author: Li, Xiao-Jun; Kim, Kwan-Woo; Oh, Hyuncheol; Liu, Xiang-Qian; Kim, Youn-Chul
Title: Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi
  • Document date: 2019_2_21
    • ID: 1vbkttzx_14
    Snippet: The proteins iNOS, COX-2, and MAPK-associated proteins, including p-p38, p38, p-JNK, JNK, p-ERK, and ERK, were detected by a Western blot analysis. The procedures of this experiment were based on our previous report [14] . Cells were lysed by RIPA buffer (Thermo Fisher Scientific, USA), and normalized for equal amounts of protein using the Bradford protein assay (Bio-Rad Laboratories, Hercules, CA, USA). 30 g of protein was loaded for each sample.....
    Document: The proteins iNOS, COX-2, and MAPK-associated proteins, including p-p38, p38, p-JNK, JNK, p-ERK, and ERK, were detected by a Western blot analysis. The procedures of this experiment were based on our previous report [14] . Cells were lysed by RIPA buffer (Thermo Fisher Scientific, USA), and normalized for equal amounts of protein using the Bradford protein assay (Bio-Rad Laboratories, Hercules, CA, USA). 30 g of protein was loaded for each sample and separated on 7.5 or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Then, proteins were transferred to nitrocellulose (NC) membranes (Bio-Rad Laboratories). After that, membranes were incubated with Tris-buffered saline containing 0.1% Tween-20 (TBS-T) with 5% skim milk (BD Difco, USA) for 1 h at 4 ∘ C. Then, membranes were probed with primary antibodies and incubated at 4 ∘ C for 90 min or overnight.

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