Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi

Li, Xiao-Jun; Kim, Kwan-Woo; Oh, Hyuncheol; Liu, Xiang-Qian; Kim, Youn-Chul

NO and PGE 2 , as well as their regulatory enzymes iNOS and COX-2, are important mediators for neuroinflammation. They are involved in the pathogenesis of various neuroinflammatory pathophysiological conditions [31] . In addition, proinflammatory cytokines, including IL-1 , IL-6, and TNF-, are important factors in CNS immune responses, and mediate neurodegeneration [3] . Microglial cells are activated by various stimuli. The activation of these cells leads to the release of these proinflammatory mediators and the exacerbation of neuroinflammation and neuroglia-induced neurotoxicity [31, 32] . Our investigation showed that compound , isolated from A. henryi, suppressed NO and PGE 2 production in LPS-stimulated BV2 microglial cells and also inhibited the LPS-induced expression of the iNOS and COX-2 protein (Figure 2) . Additionally, the present data indicated that compound significantly suppressed the release of IL-1 and TNF-, but not IL-6, at the protein and mRNA levels ( Figure 3 ).