Author: Delvecchio, Rodrigo; Higa, Luiza M.; Pezzuto, Paula; Valadão, Ana Luiza; Garcez, Patrícia P.; Monteiro, Fábio L.; Loiola, Erick C.; Dias, André A.; Silva, Fábio J. M.; Aliota, Matthew T.; Caine, Elizabeth A.; Osorio, Jorge E.; Bellio, Maria; O’Connor, David H.; Rehen, Stevens; de Aguiar, Renato Santana; Savarino, Andrea; Campanati, Loraine; Tanuri, Amilcar
Title: Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models Document date: 2016_11_29
ID: 01s21vh0_23
Snippet: Viral RNA was extracted from 200 µL supernatant of infected cells using QIAmp MiniElute Virus Spin (QIAgen, Hilden, Düsseldorf ,DE), following the manufacturer's recommendations. ZIKV detection was performed using One Step Taqman RT-qPCR (Thermo Fisher Scientific) on a 7500 Real-Time PCR System (Applied Biosystems) with primers and the probe described elsewhere [2] . Threshold cycle (CT) was determined and ∆CT (CT chloroquine treated − CT u.....
Document: Viral RNA was extracted from 200 µL supernatant of infected cells using QIAmp MiniElute Virus Spin (QIAgen, Hilden, Düsseldorf ,DE), following the manufacturer's recommendations. ZIKV detection was performed using One Step Taqman RT-qPCR (Thermo Fisher Scientific) on a 7500 Real-Time PCR System (Applied Biosystems) with primers and the probe described elsewhere [2] . Threshold cycle (CT) was determined and ∆CT (CT chloroquine treated − CT untreated) was calculated. The fold reduction of virus particles' release, including defective viral particles, were calculated by 2 ∆Ct .
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