Author: de Melo, Ivan S.; Jimenez-Nuñez, Maria D.; Iglesias, Concepción; Campos-Caro, Antonio; Moreno-Sanchez, David; Ruiz, Felix A.; Bolívar, Jorge
Title: NOA36 Protein Contains a Highly Conserved Nucleolar Localization Signal Capable of Directing Functional Proteins to the Nucleolus, in Mammalian Cells Document date: 2013_3_13
ID: 0jx6mwiw_6
Snippet: HeLa, CHO and PC3 cells (from ATCC) were cultured in Dulbecco's modified Eagle's medium at 37uC in a CO2 incubator; CHSE cells [40] were cultured in L-15 at 20uC; and S-2 (from Invitrogen) were cultured in Schneider's medium at 25uC. All the cell culture media were supplemented with 10% foetal bovine serum and 100 U/ml penicillin and 100 mg/ml streptomycin. Most of the transient transfections were performed using FuGene HD, and 1.8 mg of plasmid .....
Document: HeLa, CHO and PC3 cells (from ATCC) were cultured in Dulbecco's modified Eagle's medium at 37uC in a CO2 incubator; CHSE cells [40] were cultured in L-15 at 20uC; and S-2 (from Invitrogen) were cultured in Schneider's medium at 25uC. All the cell culture media were supplemented with 10% foetal bovine serum and 100 U/ml penicillin and 100 mg/ml streptomycin. Most of the transient transfections were performed using FuGene HD, and 1.8 mg of plasmid in 35 mm plates, according to the manufacturer's instructions. For transfections in HeLa cells with the vector pIRES 2eGFP, Lipofectamine LTX (Invitrogen) was used, according to the manufacturer's instructions.
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