Author: Cyktor, Joshua C.; Carruthers, Bridget; Beamer, Gillian L.; Turner, Joanne
Title: Clonal Expansions of CD8(+) T Cells with IL-10 Secreting Capacity Occur during Chronic Mycobacterium tuberculosis Infection Document date: 2013_3_5
ID: 1rhlu59c_10
Snippet: Mtb Erdman (ATCC 35801) was obtained from the American Type Culture Collection (Manassas, VA). Stocks were grown in Proskauer-Beck liquid medium containing 0.05% Tween 80 to mid-log phase and frozen in 1 mL aliquots at 280uC. Mice were infected with Mtb Erdman using an inhalation exposure system (Glas-Col) calibrated to deliver 50-100 CFU to the lungs of each mouse, as previously described [38] . At specific time points post Mtb infection mice we.....
Document: Mtb Erdman (ATCC 35801) was obtained from the American Type Culture Collection (Manassas, VA). Stocks were grown in Proskauer-Beck liquid medium containing 0.05% Tween 80 to mid-log phase and frozen in 1 mL aliquots at 280uC. Mice were infected with Mtb Erdman using an inhalation exposure system (Glas-Col) calibrated to deliver 50-100 CFU to the lungs of each mouse, as previously described [38] . At specific time points post Mtb infection mice were sacrificed and lungs were aseptically removed into sterile saline. Organs were homogenized and serial dilutions plated onto 7H11 agar supplemented with OADC as previously described [4] . Plates were incubated at 37uC for 21 days in order to enumerate bacterial colonies and calculate the bacterial burden.
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