Bayesian mixture analysis for metagenomic community profiling.

Sofia Morfopoulou; Vincent Plagnol

Following the initial processing, we used metaMix for species identification and abundance estimation. The resulting species profile is shown in Table 4 ; the 13 metaMix entries correspond to 10 species. The most abundant organism was the φX174 bacteriophage, which is routinely used for deep-sequencing quality control. More interestingly, we identified an astrovirus. Five short assembled contigs (44 reads) with length ranging between 167bp and 471bp and two non-assembled reads were assigned to the Astrovirus VA1 with a probability score of 1 (Figure 2) . metaMix also identified a number of bacteria supported by a few reads. These are either known laboratory reagent contaminants or human skin associated contaminants (Salter et al., 2014) . The analysis completed in 29 minutes.