Selected article for: "cycle threshold and nucleic acid extraction"
Author: Yu, Fei; Qiu, Ting; Zeng, Ying; Wang, Yiyin; Zheng, Shufa; Chen, Xiao; Chen, Yu
Title: Comparative Evaluation of Three Preprocessing Methods for Extraction and Detection of Influenza A Virus Nucleic Acids from Sputum
  • Document date: 2018_3_2
    • ID: 0kt9b1ww_11
    Snippet: RNA was extracted simultaneously everyday by using the Automated Nucleic Acid Extraction System through a paramagnetic beads method (Zhijiang Biotechnology Co., Ltd., Shanghai, China). The concentration and purity (A260/A280 ratio) of nucleic acid for each RNA extract were evaluated with a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). A260/A280 ratio of pure RNA products ranged from 1.8 to 2.0 (8). Extracted RNA.....
    Document: RNA was extracted simultaneously everyday by using the Automated Nucleic Acid Extraction System through a paramagnetic beads method (Zhijiang Biotechnology Co., Ltd., Shanghai, China). The concentration and purity (A260/A280 ratio) of nucleic acid for each RNA extract were evaluated with a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). A260/A280 ratio of pure RNA products ranged from 1.8 to 2.0 (8). Extracted RNA was tested by rRT-PCR using IAV Detection kit (Zhijiang Biotechnology Co., Ltd.) with ABI 7500 Real-Time PCR instrument (Foster City, CA, USA). Threshold cycle (Ct) values being negatively related to concentration of nucleic acid were recorded. Operation and result assessment were conducted in accordance with the manufacturers' instructions. Qualified negative, positive, and internal controls were the premise of results validity.

    Search related documents:
    Co phrase search for related documents
    • Automated Nucleic Acid Extraction System and Ct value: 1
    • Automated Nucleic Acid Extraction System and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • Automated Nucleic Acid Extraction System and Real time: 1, 2, 3, 4, 5
    • bead method and nucleic acid: 1
    • bead method and Real time: 1, 2
    • Ct value and internal control: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • Ct value and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • Ct value and PCR instrument: 1, 2
    • Ct value and Real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • Ct value and Real time PCR instrument: 1
    • internal control and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • internal control and PCR instrument: 1
    • internal control and Real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • manufacturer instruction and nucleic acid: 1
    • manufacturer instruction and Real time: 1
    • nucleic acid and PCR instrument: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • nucleic acid and purity concentration: 1, 2, 3
    • nucleic acid and Real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • nucleic acid and Real time PCR instrument: 1, 2, 3