Selected article for: "cell proliferation and increase proliferation"
Author: Marriott, Andrew S.; Vasieva, Olga; Fang, Yongxiang; Copeland, Nikki A.; McLennan, Alexander G.; Jones, Nigel J.
Title: NUDT2 Disruption Elevates Diadenosine Tetraphosphate (Ap(4)A) and Down-Regulates Immune Response and Cancer Promotion Genes
  • Document date: 2016_5_4
    • ID: 0ozzbp85_61
    Snippet: Transcript stability. An alternative mechanism whereby increased Ap 4 A could have a major effect on the transcriptome is through inhibition of RNA binding by the nudix protein NUDT21 (CFIm25, CPSF5), the 25 kDa component of the cleavage factor Im complex involved in pre-mRNA 3 0 -end processing. Ap 4 A binds to the same site as RNA with a K d of 2.4 μM and so might be expected to affect 3 0 -end processing and the half-lives of certain mRNAs [3.....
    Document: Transcript stability. An alternative mechanism whereby increased Ap 4 A could have a major effect on the transcriptome is through inhibition of RNA binding by the nudix protein NUDT21 (CFIm25, CPSF5), the 25 kDa component of the cleavage factor Im complex involved in pre-mRNA 3 0 -end processing. Ap 4 A binds to the same site as RNA with a K d of 2.4 μM and so might be expected to affect 3 0 -end processing and the half-lives of certain mRNAs [35, 149] . Knockdown of NUDT21 in glioblastoma cells results in shortened 3 0 -UTRs in 1450 transcripts and an increase in cell proliferation [150] . Of these 1450 transcripts, the steady-state levels of 928 were significantly increased and 28 were decreased. However, a comparison between the transcripts affected after NUDT21 knockdown in glioblastoma cells and those up-and down-regulated in NuKO cells revealed no overlap between the down-regulated genes and only 1 overlap in the top 250 up-regulated genes. Therefore, despite the differences in cell lines, it seems unlikely that NUDT21 is an important target for the increased Ap 4 A in NuKO cells.

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