Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer’s patches

Yuan, Chen; Zhang, En; Huang, Lulu; Wang, Jialu; Yang, Qian

Van Kruiningen et al.noted that the PPs occupied most of the area in the ileum [20, 21] . Within PPs, DCs in the dome and interfollicular areas can uptake antigens from the gut lumen by extending dendrites into epithelium, then and process and present to lymphocytes, which effectively antigen produces an immune response [4, 14, 15] . DCs derived from blood and bone marrow precursors can't represent the real immune status of the body. Mice are the most commonly used animal models, but because of their small size they have a low amount of DCs in their intestinal mucosal. Moreover, the immune system of mice have less than 10% similarity to humans [22] . In addition, it is difficult to identify and collect DCs from human PPs, study of DCs from human PPs is difficult to achieve [2] . Therefore, the current study is about the isolation of DCs in porcine PPs. Pigs are very similar in anatomy, physiology and genetics to humans. Moreover, the immune system of pigs is over 80% similar to humans [22] [23] [24] . A large number of experiments have proved that pigs can be used as an ideal experimental animal model for the establishment of human disease infection and can be used to isolate and characterize the DCs in porcine PPs [25] [26] [27] . So, it is important to establish a method to isolate a number of functional DCs in vitro. In this study, we establish a method to isolate DCs in porcine PPs, which could contribute to further study on the role of DCs in porcine PPs.