Author: Steelman, Andrew J; Li, Jianrong
Title: Poly(I:C) promotes TNFa/TNFR1-dependent oligodendrocyte death in mixed glial cultures Document date: 2011_8_3
ID: 16032h3d_7
Snippet: Primary cells (mono-, co-, and mixed glial cultures as well as splenocytes) were isolated from Sprague Dawley rats (Harland; Houston TX). Mouse cells were obtained from wildtype B6.129SF2/J, C57BL/6J, and TNFα -/-(background B6.129SF2/J) and TNFR1 -/-(background C57BL/6J) mice from The Jackson Laboratory (Bar Harbor, ME). Animals were housed under constant 12 h light/dark cycles in covered cages and fed with a standard rodent diet ad libitum. Al.....
Document: Primary cells (mono-, co-, and mixed glial cultures as well as splenocytes) were isolated from Sprague Dawley rats (Harland; Houston TX). Mouse cells were obtained from wildtype B6.129SF2/J, C57BL/6J, and TNFα -/-(background B6.129SF2/J) and TNFR1 -/-(background C57BL/6J) mice from The Jackson Laboratory (Bar Harbor, ME). Animals were housed under constant 12 h light/dark cycles in covered cages and fed with a standard rodent diet ad libitum. All experimental procedures were approved by the Institutional Animal Care and Use Committee and were performed in accordance with guidelines of the National Institutes of Health.
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