Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2 Document date: 2019_1_17
ID: 1c5ug64m_11
Snippet: Polymerase chain reaction was set up by adding 10 μL Premix Taq™ (TaKaRa Taq™ Version 2.0) (TaKaRa, Dalian, China), 1 μL PCR primers (10 μM each), 1 μL DNA template and the reaction volume was made up to 20 μL using nuclease free ddH 2 O. PCR amplification was performed with an initial denaturation step of 94°C for 5 min, 35 cycles of denaturation at 94°C for 20 s, annealing at 50°C for 20 s, and elongation at 72°C for 20 s, followed.....
Document: Polymerase chain reaction was set up by adding 10 μL Premix Taq™ (TaKaRa Taq™ Version 2.0) (TaKaRa, Dalian, China), 1 μL PCR primers (10 μM each), 1 μL DNA template and the reaction volume was made up to 20 μL using nuclease free ddH 2 O. PCR amplification was performed with an initial denaturation step of 94°C for 5 min, 35 cycles of denaturation at 94°C for 20 s, annealing at 50°C for 20 s, and elongation at 72°C for 20 s, followed by a final extension step of 72°C for 8 min. characterised to assess the antigenic type according to previous report [33] .
Search related documents:
Co phrase search for related documents- denaturation step and extension step: 1
- denaturation step and PCR amplification: 1, 2
- denaturation step and PCR primer: 1, 2
- dna template and extension step: 1, 2
- dna template and PCR amplification: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
- dna template and PCR primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
- dna template and polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- dna template and reaction volume: 1, 2, 3
- extension step and PCR amplification: 1, 2
- extension step and PCR primer: 1, 2, 3, 4
- extension step and polymerase chain reaction: 1, 2, 3
- extension step and reaction volume: 1
- PCR amplification and polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- PCR amplification and reaction volume: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- PCR primer and polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49
- PCR primer and reaction volume: 1, 2, 3, 4, 5, 6
- polymerase chain reaction and previous report: 1, 2, 3, 4, 5
- polymerase chain reaction and reaction volume: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- previous report and reaction volume: 1
Co phrase search for related documents, hyperlinks ordered by date