Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2 Document date: 2019_1_17
ID: 1c5ug64m_19
Snippet: Purified PCR products were tested using the LFIA to determine the optimal final reaction volume and time (Fig. 2) . To achieve maximal fluorescence signal on test line, we optimized the working reaction volume in the range of 60 μL-140 μL. As shown in Fig. 2 , with the increasing of working reaction volume, the fluorescence signals reached the highest point in 100 μL and maintained stably till 140 μL (Fig. 2a and c) . In addition, the fluores.....
Document: Purified PCR products were tested using the LFIA to determine the optimal final reaction volume and time (Fig. 2) . To achieve maximal fluorescence signal on test line, we optimized the working reaction volume in the range of 60 μL-140 μL. As shown in Fig. 2 , with the increasing of working reaction volume, the fluorescence signals reached the highest point in 100 μL and maintained stably till 140 μL (Fig. 2a and c) . In addition, the fluorescence signals reached the highest point with incubating for 120 s at room temperature and slight difference in band clarity was observed with increasing reaction time from 120 s to 180 s ( Fig. 2b and d) . The results showed that the best reaction condition was 100 μL working reaction volume incubating for 120 s (2 min) at room temperature, and thus was chosen for the following experiments.
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