Selected article for: "cycle threshold and real time pcr"
Author: Muñoz-González, Sara; Ruggli, Nicolas; Rosell, Rosa; Pérez, Lester Josué; Frías-Leuporeau, Maria Teresa; Fraile, Lorenzo; Montoya, Maria; Cordoba, Lorena; Domingo, Mariano; Ehrensperger, Felix; Summerfield, Artur; Ganges, Llilianne
Title: Postnatal Persistent Infection with Classical Swine Fever Virus and Its Immunological Implications
  • Document date: 2015_5_4
    • ID: 13cii3on_8
    Snippet: RNA was extracted from all the samples using the NucleoSpin RNA isolation kit (Macherey-Nagel), according to the manufacturer's instructions. In all cases, RNA was extracted from an initial sample volume of 150 μL to obtain a final volume of 50 μL of RNA, which was stored at -80°C. The presence of CSFV RNA in the serum and in nasal and rectal swabs, as well as tonsil, thymus and bone marrow samples, was analysed by real time (RT)-PCR [36] . Th.....
    Document: RNA was extracted from all the samples using the NucleoSpin RNA isolation kit (Macherey-Nagel), according to the manufacturer's instructions. In all cases, RNA was extracted from an initial sample volume of 150 μL to obtain a final volume of 50 μL of RNA, which was stored at -80°C. The presence of CSFV RNA in the serum and in nasal and rectal swabs, as well as tonsil, thymus and bone marrow samples, was analysed by real time (RT)-PCR [36] . This test was used in our laboratory for inter-laboratory comparisons of CSFV diagnoses, organised by the EU Reference Laboratory. Positive results were considered for threshold cycle values (CT) equal to or less than 42. Samples in which fluorescence was undetectable were considered negative.

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