Author: Le-Viet, Nhiem; Le, Viet-Nho; Chung, Hai; Phan, Duc-Tuan; Phan, Quang-Duong; Cao, Thanh-Van; Abat, Cédric; Raoult, Didier; Parola, Philippe
Title: Prospective case-control analysis of the aetiologies of acute undifferentiated fever in Vietnam Document date: 2019_3_4
ID: 0uwm4dk9_59
Snippet: For each patient, 200 µL of whole blood and 200 µL of urine were used for DNA extraction, and 200 µL of acute-phase plasma and 200 µL of the throat swab sample in viral transport media were used for DNA/ RNA extraction. The eschar specimens were pretreated and used for DNA extraction as previously described [26] . DNA extraction using the DNA EZ1 extraction kit and DNA/RNA extraction using the EZ1 Virus Mini Kit v2.0 (Qiagen, Hilden, Germany).....
Document: For each patient, 200 µL of whole blood and 200 µL of urine were used for DNA extraction, and 200 µL of acute-phase plasma and 200 µL of the throat swab sample in viral transport media were used for DNA/ RNA extraction. The eschar specimens were pretreated and used for DNA extraction as previously described [26] . DNA extraction using the DNA EZ1 extraction kit and DNA/RNA extraction using the EZ1 Virus Mini Kit v2.0 (Qiagen, Hilden, Germany) were performed with the EZ1 Advanced XL Robot (Qiagen) according to the manufacturer's instructions. The DNA and DNA/RNA products were either immediately used or stored at −20°C and at −80°C until molecular analysis, respectively. To avoid crosscontamination, the nucleic acid-extracting EZI Advanced XL Robot was disinfected after each batch of extraction based on the manufacturer's recommendations.
Search related documents:
Co phrase search for related documents- Mini kit and RNA extraction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49
- Mini kit and swab sample: 1
- Mini kit and transport medium: 1, 2, 3, 4
- Mini kit and viral transport medium: 1, 2, 3
- Mini kit and Virus Mini kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- molecular analysis and RNA extraction: 1, 2, 3, 4
- molecular analysis and swab sample: 1
- molecular analysis and transport medium: 1, 2
- molecular analysis and viral transport medium: 1
- RNA extraction and swab sample: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- RNA extraction and transport medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42
- RNA extraction and viral transport medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- RNA extraction and Virus Mini kit: 1, 2, 3, 4, 5
- swab sample and transport medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- swab sample and viral transport medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- swab sample and viral transport medium swab sample: 1, 2
- transport medium and viral transport medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77
- transport medium and viral transport medium swab sample: 1, 2
- transport medium and Virus Mini kit: 1
Co phrase search for related documents, hyperlinks ordered by date