Author: Cong, Yingying; Kriegenburg, Franziska; de Haan, Cornelis A. M.; Reggiori, Fulvio
Title: Coronavirus nucleocapsid proteins assemble constitutively in high molecular oligomers Document date: 2017_7_18
ID: 15hzah62_27
Snippet: MHV-infected LR7 cells were centrifuged at 15,000 × g for 10 min (4 °C) to obtain a pellet (P13) and a supernatant (S13), which was further centrifuged at 110,000 × g for 60 min (4 °C) to also get a pellet (P45) and a supernatant (S45). Proportional aliquots of Ext, P13, P45 and S45 fractions were examined by resolving them by SDS-PAGE and then by probing western blot membranes with monoclonal antibodies against MHV N protein and polyclonal a.....
Document: MHV-infected LR7 cells were centrifuged at 15,000 × g for 10 min (4 °C) to obtain a pellet (P13) and a supernatant (S13), which was further centrifuged at 110,000 × g for 60 min (4 °C) to also get a pellet (P45) and a supernatant (S45). Proportional aliquots of Ext, P13, P45 and S45 fractions were examined by resolving them by SDS-PAGE and then by probing western blot membranes with monoclonal antibodies against MHV N protein and polyclonal antisera against GAPDH (Fitzgerald, North Acton, MA), tubulin (Sigma-Aldrich, St. Louis, MO) or VAPA (Santa Cruz, Dallas, TX).
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