Redox process is crucial for inhibitory properties of aurintricarboxylic acid against activity of YopH: virulence factor of Yersinia pestis

Kuban-Jankowska, Alicja; Sahu, Kamlesh K; Niedzialkowski, Pawel; Gorska, Magdalena; Tuszynski, Jack A; Ossowski, Tadeusz; Wozniak, Michal

In conclusion, we demonstrated that ATA effectively decreases the Yersinia YopH activity and has stronger binding to YopH active site than natural substrate. Here we proposed a unique mechanism of ATA caused inactivation of YopH involved with its redox process leading to generation of hydrogen peroxide. We suggest that the potent inhibitory properties of ATA are due to strong binding in the YopH active site and generation of hydrogen peroxide directly inside the active site. The studies on inhibitory properties of ATA against YopH are a good starting point for the synthesis of analogous inhibitors and potentially lead to clinical applications. Further studies on ATA analogs and cellular aspects of ATA are underway. www.impactjournals.com/oncotarget