Revalidation and genetic characterization of new members of Group C (Orthobunyavirus genus, Peribunyaviridae family) isolated in the Americas

Nunes, Márcio Roberto Teixeira; de Souza, William Marciel; Acrani, Gustavo Olszanski; Cardoso, Jedson Ferreira; da Silva, Sandro Patroca; Badra, Soraya Jabur; Figueiredo, Luiz Tadeu Moraes; Vasconcelos, Pedro Fernando da Costa

Maximum likelihood (ML) phylogenetic trees were constructed using nucleotide and amino acids sequences of viruses reported in our study and additional sequences of members of Group C viruses with complete coding sequences (S, M, and L) available in the GenBank database (http://www.ncbi.nlm.nih.gov/) until 10 th of April of 2018. The MSAs were carried out using Muscle v.3.7 [15] . The phylogenies were inferred by IQ-TREE version 1.4.3 software using the best-fit model based on Bayesian Information Criterion, and the GTR+I+G4 nucleotide substitution model was used to all RNA segments, and LG+G4, LG+I+G4, and LG+G4 amino acids substitution model to S, M, and L, respectively. Statistical supports for individual nodes were estimated using the bootstrap value using 1,000 replicates [16] . The phylogenetic trees were visualized using the FigTree software v.1.4.2. In addition, the nucleotides and amino acid distances among viruses of Group C were estimated with segment S, M, and L using the p-distance values. Standard error estimations were calculated by bootstrapping method (1,000 replicates) using the MEGA v.6 program [17] .