Revalidation and genetic characterization of new members of Group C (Orthobunyavirus genus, Peribunyaviridae family) isolated in the Americas

Nunes, Márcio Roberto Teixeira; de Souza, William Marciel; Acrani, Gustavo Olszanski; Cardoso, Jedson Ferreira; da Silva, Sandro Patroca; Badra, Soraya Jabur; Figueiredo, Luiz Tadeu Moraes; Vasconcelos, Pedro Fernando da Costa

MURV strain BeAn974, ORIV strain BeAn17, MTBV strain BeAn15, CARV strain BeAn3994 and MADV strain BT4075 viruses present 99.96 to 100% nucleotide identities with the corresponding partial genomic sequences that were previously reported (Fig 3D-3F and Parts D-F of S1 Fig) [4, 7, 8 ]. On the other hand, the new viruses that we have been sequenced the complete coding sequences shared higher nucleotide identify with partial genomes, such as Apeu strain BeAn 848, which shared 99% nucleotide identify in all segments with partial sequences of same strains previously described [9] . In addition, the amino acid substitutions were observed as follow: CARV strain BeAn3994 (S344P and K605N) on GPC. MTBV strain BeAn15 (Q335R); (L728Q); (Q1073K) and (K1345R) on RdRp and (G456R and D626N) in GPC. MURV strain BeAn974 (F949S) in RdRp. However, we did not observe any amino acid substitutions in the nucleoprotein sequence of the viruses that were submitted to the resequencing protocol. On the other hand, the identified amino acids substitutions observed in GPC and RdRp of our results compared with previously sequences reported to same strains. Therefore, we suspected that this fact is probably due to serial passages in cultured cells [26] . Collectively, our results are consistent with the same viral strains sequenced previously [4, [7] [8] [9] , but the results of segment S suggested that some complete coding sequences previously reported for Group C viruses are possibly are incomplete.