Author: Nunes, Márcio Roberto Teixeira; de Souza, William Marciel; Acrani, Gustavo Olszanski; Cardoso, Jedson Ferreira; da Silva, Sandro Patroca; Badra, Soraya Jabur; Figueiredo, Luiz Tadeu Moraes; Vasconcelos, Pedro Fernando da Costa
Title: Revalidation and genetic characterization of new members of Group C (Orthobunyavirus genus, Peribunyaviridae family) isolated in the Americas Document date: 2018_5_24
ID: 17f1evh3_7
Snippet: Viral RNAs were extracted from the supernatant of infected VERO cells using the PureLink Viral RNA Mini Kit (Invitrogen, USA) following the manufactures instructions. Then, the strands of the RNA synthesis were performed using the kit cDNA Synthesis System and 400 μM Roche "random" Primer according to the manufacturer's instructions. The cDNAs were prepared for HTS on a GS FLX+ pyrosequencer (Roche, 454 Life Sciences) at the Center for Technolog.....
Document: Viral RNAs were extracted from the supernatant of infected VERO cells using the PureLink Viral RNA Mini Kit (Invitrogen, USA) following the manufactures instructions. Then, the strands of the RNA synthesis were performed using the kit cDNA Synthesis System and 400 μM Roche "random" Primer according to the manufacturer's instructions. The cDNAs were prepared for HTS on a GS FLX+ pyrosequencer (Roche, 454 Life Sciences) at the Center for Technological Innovation at the Evandro Chagas Institute, Ministry of Health, Brazil. The de novo assembling strategy applied to obtain the genomes was used with program Newbler v.
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