Author: Guo, Jiao; Jia, Xiaoying; Liu, Yang; Wang, Shaobo; Cao, Junyuan; Zhang, Bo; Xiao, Gengfu; Wang, Wei
Title: Screening of Natural Extracts for Inhibitors against Japanese Encephalitis Virus Infection Document date: 2020_2_21
ID: 161ck1i9_1
Snippet: Optimization of HCS assay conditions. The HCS assay-optimized conditions contain cell density, infective dose, and assay endpoint. The assay conditions for HCS were optimized as follows: cell density of 10,000 cells per 96-well plate, a multiplicity of infection (MOI) of 0.8, and an endpoint of 24 h postinfection. The signal-to-basal (S/B) ratio, coefficient of variation (CV), and Z factor were 2,177, 8.9%, and 0.72, respectively, which demonstra.....
Document: Optimization of HCS assay conditions. The HCS assay-optimized conditions contain cell density, infective dose, and assay endpoint. The assay conditions for HCS were optimized as follows: cell density of 10,000 cells per 96-well plate, a multiplicity of infection (MOI) of 0.8, and an endpoint of 24 h postinfection. The signal-to-basal (S/B) ratio, coefficient of variation (CV), and Z factor were 2,177, 8.9%, and 0.72, respectively, which demonstrated that the assay was robust and reproductive for the large-scale screening of novel antiviral compounds against JEV infection.
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