Author: Zhang, Chi; Xiu, Leshan; Xiao, Yan; Xie, Zhengde; Ren, Lili; Peng, Junping
Title: Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass Spectrometry Document date: 2018_4_5
ID: 0k2blp7n_26
Snippet: Samples determined pathogen-positive by the BP-MS method were re-tested first by real-time PCR. Using a combination of multiplex and singleplex real-time PCRs, 46.1% (77/167) of the positive samples showed results concordant with Table 3 . Thirty-one BP-MS-positive samples were not verified by either real-time PCR or nested PCR. Of these, 14 were single-infection samples and 17 were multiple-infection samples......
Document: Samples determined pathogen-positive by the BP-MS method were re-tested first by real-time PCR. Using a combination of multiplex and singleplex real-time PCRs, 46.1% (77/167) of the positive samples showed results concordant with Table 3 . Thirty-one BP-MS-positive samples were not verified by either real-time PCR or nested PCR. Of these, 14 were single-infection samples and 17 were multiple-infection samples.
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