Author: Fan, Qing; Kopp, Sarah J.; Connolly, Sarah A.; Longnecker, Richard
Title: Structure-Based Mutations in the Herpes Simplex Virus 1 Glycoprotein B Ectodomain Arm Impart a Slow-Entry Phenotype Document date: 2017_5_16
ID: 1v6nf28a_15
Snippet: Previously, mutations in the C-terminal region of the gB ectodomain (the domain V arm) demonstrated that this region of gB is important for fusion function (20) . Triple alanine mutations (I671A, H681A, and F683A) in this gB arm region resulted in decreased cell-cell and virus-cell fusion, despite WT levels of cell surface expression and monoclonal antibody (MAb) reactivity (20) . To investigate the effect of the triple alanine mutations on virus.....
Document: Previously, mutations in the C-terminal region of the gB ectodomain (the domain V arm) demonstrated that this region of gB is important for fusion function (20) . Triple alanine mutations (I671A, H681A, and F683A) in this gB arm region resulted in decreased cell-cell and virus-cell fusion, despite WT levels of cell surface expression and monoclonal antibody (MAb) reactivity (20) . To investigate the effect of the triple alanine mutations on virus entry, we constructed HSV-1 gB 3A viruses carrying these mutations by using a BAC (32, 33) and characterized the entry of these viruses into cells.
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