Author: Fan, Qing; Kopp, Sarah J.; Connolly, Sarah A.; Longnecker, Richard
Title: Structure-Based Mutations in the Herpes Simplex Virus 1 Glycoprotein B Ectodomain Arm Impart a Slow-Entry Phenotype Document date: 2017_5_16
ID: 1v6nf28a_34
Snippet: Multiple-step growth curve analysis. Multiple-step growth curves were determined as previously described (55) . Vero cell monolayers in six-well plates were infected with HSV-1 gB 3A or GS3217 (WT) virus at 0.01 PFU/cell for 1 h. The inoculum was removed, and the cells were rinsed twice with PBS and then incubated at 37°C (time zero). At 0, 12, 24, 36, and 48 h of incubation, cells were harvested and freeze-thawed at Ϫ40°C three times and viru.....
Document: Multiple-step growth curve analysis. Multiple-step growth curves were determined as previously described (55) . Vero cell monolayers in six-well plates were infected with HSV-1 gB 3A or GS3217 (WT) virus at 0.01 PFU/cell for 1 h. The inoculum was removed, and the cells were rinsed twice with PBS and then incubated at 37°C (time zero). At 0, 12, 24, 36, and 48 h of incubation, cells were harvested and freeze-thawed at Ϫ40°C three times and virus titers were determined by plaque assay on Vero cells.
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