Author: Fan, Qing; Kopp, Sarah J.; Connolly, Sarah A.; Longnecker, Richard
Title: Structure-Based Mutations in the Herpes Simplex Virus 1 Glycoprotein B Ectodomain Arm Impart a Slow-Entry Phenotype Document date: 2017_5_16
ID: 1v6nf28a_37
Snippet: Plaque size determination after gB mutant complementation. Vero cells in six-well plates were transfected with 1,500 ng of the vector or a plasmid encoding gB 3A or gB 876T. After 24 h, cells were infected with about 200 PFU of gB 3A-1 or gB 3A-2 virus for 2 h, followed by methylcellulose overlay. After 3 days of incubation, plaques were visualized by Giemsa staining and imaged by transmitted-light microscopy with an EVOS Cell Imaging System. The.....
Document: Plaque size determination after gB mutant complementation. Vero cells in six-well plates were transfected with 1,500 ng of the vector or a plasmid encoding gB 3A or gB 876T. After 24 h, cells were infected with about 200 PFU of gB 3A-1 or gB 3A-2 virus for 2 h, followed by methylcellulose overlay. After 3 days of incubation, plaques were visualized by Giemsa staining and imaged by transmitted-light microscopy with an EVOS Cell Imaging System. The radii of at least 40 randomly selected plaques were measured, and the plaque area was calculated on the basis of the radii.
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