Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p Document date: 2014_8_13
ID: 1i6hni9s_17
Snippet: The cell lysates were prepared as described previously [24] . Western blot analysis was performed using primary antibodies against inducible nitric oxide synthase (iNOS) (0.1 μg/ml), TNFα (0.1 μg/ml), IL-1β (0.1 μg/ml), TGFβ (0.125 μg/ml), CD14 (0.1 μg/ml), soluble tumor necrosis factor receptor (sTNFR) type II (2 μg/ml), IL-1RA (2.5 μg/ml) (Abcam, Cambridge, MA, USA), or CD68 (0.9 μg/ml; Epitomics, Burlingame, CA, USA). The density of.....
Document: The cell lysates were prepared as described previously [24] . Western blot analysis was performed using primary antibodies against inducible nitric oxide synthase (iNOS) (0.1 μg/ml), TNFα (0.1 μg/ml), IL-1β (0.1 μg/ml), TGFβ (0.125 μg/ml), CD14 (0.1 μg/ml), soluble tumor necrosis factor receptor (sTNFR) type II (2 μg/ml), IL-1RA (2.5 μg/ml) (Abcam, Cambridge, MA, USA), or CD68 (0.9 μg/ml; Epitomics, Burlingame, CA, USA). The density of protein bands was assessed using a computing densitometer with Image-Pro plus software (Media Cybernetics, Inc., Bethesda, MD, USA).
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